537 How solar exposure affect skin ecosystem interactions and SPF50+ sunscreen afford innovative photoprotection: a multi-omics approach

Skin is the primary interface with external environment. To evaluate impact of factors like sun exposure on cutaneous ecosystem (skin-sebum-microbiota), it has to be studied as a whole understand how they interact each other. We developed reconstructed human epidermal model colonized microbiota and sebum reproduce complexity skin ecosystem. This in vitro was exposed simulated solar radiation (SSR). A new SPF50+ photoprotective system containing specific combination four filters (TriAsorB, Tinosorb S, Uvinul T150 A+) affording broad spectrum UVB+A photoprotection, evaluated this model. Metabolomic profiles were performed NMR UHPLC-HRMS. Metagenomic analyses from genomic DNA extract samples. ITS1 ribosomal RNA gene, V1-V3 region 16S gene sequenced by high-speed sequencing. Based metabolomic analyses, under high oxidative stress modification glutathione purine pathway but also urea cycle. Other pathways have been highlighted linked microbiota, BCAAs cycle tryptophan pathway. With system, metabolome preserved physiological interactions within [DH1] protected deleterious effects. study identified highly accurate signature an complete correlated metagenomic analysis explain between microbiota.